Perithecia (85–)110–150(–170) × (100–)110–150(–185) μm (n = 30), flask-shaped or globose, usually not crowded; peridium yellowish, (8–)10–14(–18) μm (n = 60) thick at the base and sides. Cortical layer (3–)4–13(–19) μm (n = 30) thick, consisting of a hyaline t. intricata of narrow, thin-walled hyphae (1.2–)2.0–3.2(–4.3)

μm (n = 40) wide, often spiral at the surface, and of an incomplete cellular cortex present in pigmented areas, of cells (5–)7–13(–15) × (3–)4–9(–12) μm (n = 30) in face view; often covered by yellow(-brown) amorphous material; no find more subcortical tissue differentiated. Subperithecial tissue a hyaline t. intricata of AZD6094 research buy thin-walled hyphae (2.5–)3–6(–7) μm (n = 40) wide, merging into a t. angularis–epidermoidea of hyaline, thin-walled, isodiametric to oblong cells (3–)4–8(–11) × (2.5–)3–6(–9) selleck chemical μm (n = 30) in discontinuous areas close to the host. Asci (40–)47–67(–77) × (2.7–)3.3–5.0(–6.0) μm, stipe (1–)3–11(–20) μm long (n = 127); apex truncate, with a flat ring below

the apical thickening; no croziers seen. Ascospores hyaline, smooth inside the asci, finely verruculose after ejection, verrucose in cotton blue/lactic acid; cells monomorphic, (sub-)globose; distal cell (2.0–)2.5–3.5(–4.0) μm diam, l/w 0.9–1.1(–1.2); proximal cell (2.0–)2.5–3.5(–4.5) μm diam, l/w (0.8–)0.9–1.1(–1.3) (n = 181). Stroma margins often bearing conidiophores (1–)2–3.5 μm wide, with sinuous ends and sparse, narrow, subulate phialides and minute globose conidial heads 10–15 μm diam. Conidia (3.5–)4.0–5.7(–7.5) × (2.0–)2.5–3.0(–3.4) Metalloexopeptidase μm, l/w (1.2–)1.5–2.1(–2.6) (n = 78), oblong-cylindrical or ellipsoidal, hyaline, smooth. Cultures and anamorph: optimal growth at 25°C on all media, negligible growth at 30°C, no growth at 35°C.

On CMD after 72 h 17–22 mm at 15°C, 36–46 mm at 25°C, 0.5–1 mm at 30°C; mycelium covering the plate after 5 days at 25°C. Colony hyaline to pale yellowish or greyish orange, 5A2, 5B3, after 3 weeks, thin, indistinctly zonate, mycelium dense, with radial streaks; primary surface hyphae conspicuously thick and coarsely wavy; mycelial aggregations and long aerial hyphae appearing along the margin, sometimes forming white cottony spots. No conidiation seen within 7 weeks. No autolytic excretions noted. Coilings moderate. No distinct odour noted. Chlamydospores frequent, terminal and intercalary, noted after 3–6 days at 25°C. On PDA after 72 h 15–17 mm at 15°C, 31–36 mm at 25°C, 0.3–0.6 mm at 30°C; mycelium covering the plate after 1 weeks at 25°C. Colony circular, thin, zonate, hairy. Margin shiny, thin and smooth. Mycelium densely agglutinated, appearing glassy, primary surface hyphae conspicuously wide.