However, the 50-year differences are so large and occurred so uniformly in all six study areas, that a misinterpretation of trends can be excluded. Moreover, the direct comparison of historical and current maps (see Fig. 2) supports the data presented in Tables 2, 3 and 4. Soons et al. (2005), who investigated changes in Dutch moist and wet grasslands since 1900, came to similar conclusions. They found the largest reduction in patch size (AM) during the first half of the twentieth century, with an average reduction by 0.2 ha per year over the last 100 years. Two of our study areas (Helme and Nuthe) showed a larger effective Pictilisib in vitro mesh size (MESH) in 2008 than the other areas.

At these sites, wet meadows covered a particularly large area in the 1950/1960s which seems to have retarded fragmentation in the past 50 years. Large patches of meadow vegetation generally harbour LY2874455 in vitro a larger proportion of the species pool since edge effects are reduced

(Kiviniemi and Eriksson 2002). A high connectivity of meadow localities in historical time may also have a positive effect on the species richness of temperate grasslands in recent time (Lindborg and Eriksson 2004). In addition, many typical wet meadow species are adapted to seed dispersal by flooding (Gerard et al. 2008). Given that Central European river floodplains nowadays are less frequently flooded than in the past, the probability of natural seed input from abroad is most likely smaller in remnant areas that are small and isolated than in large patches. In addition, isolated meadow patches of small size will expose Tideglusib their plant populations to the increased risks of genetic drift and the harmful consequences of stochastic population fluctuations that may eventually lead to their extinction. Local and continent-wide drivers of vegetation change Substantial area losses were also recorded in the protected Havel floodplains, in particular in the species-rich mesic meadows, which demonstrates that the existing legislative tools for nature protection are not sufficient in the agricultural landscape, because they allowed a certain degree of agricultural intensification, at least

in the years before 1990. In most nature reserves dedicated to protect species-rich meadows, it is nowadays prohibited to intensify agricultural management, but this does not exclude effects of atmospheric N deposition, nutrient input through sedimentation processes (Gulati and van Donk 2002), and climatic changes, which act as additional large-scale drivers of vegetation change in both unprotected and protected meadow areas. Despite these overarching threats, the Havel example demonstrates that protection efforts were successful in GSK126 preserving a large patch of species-rich wet and mesic meadows with sufficient connectivity of the localities in the landscape. In most parts of north Germany and also in the Netherlands (Soons et al.