H, C, O stable isotope ratios and the content of 9 elements (Cu, Cr, Ni, Zn, Pb, Co, As, Cd, and U) of 31 Romanian apple juices collected from four Transylvanian 17-AAG cost areas are presented and discussed in this study.2. ExperimentalOrganic apple samples were collected in the period from august 27, 2010 to September 19,2010 from different Transylvanian areas. Fruits were taken from trees, shipped in the lab, and squeezed for a week after picking them up. The variety of investigated apple was quite large; we analyzed different apple sorts like: Jonathan, Starkrimson, Golden, Melba, Legana, Classic Pippen, Stark Earliest, Voinea, and Generos of Romania, from four Transylvanian areas.Transylvania is a Romanian province situated in the north-west towards the centre of the country, being surrounded by the Carpathians; the natural framework is made up of mountains, rivers, and plateaus.

In Transylvania, the temperature can reach +35��C in summer and ?25��C in winter. The average annual temperature is about 6�C8��C and the average annual rainfall about 700�C1000mm/year.2.1. Stable Isotope Analysis2.1.1. Sample Preparation For oxygen-18 determination, 5mL of raw juice (neither centrifuged nor filtered) was equilibrated with CO2 for 15 hours according to the CEN:ENV 13141:1997 method at 25 �� 0.1��C [2]. The carbon dioxide was then extracted and purified. For the hydrogen analysis, a distiller under static vacuum was used with ��Rittenberg trousers�� on 2-3mL of fruit juice, always with the quantitative recovery of the water [12].

For ��13C analysis, the separation and purification of the pulp was made according to [6, 7] by the separation of a sample of about 50mL of fruit juice by centrifugation (10min Batimastat at 1400 times g) from the pulp. The pulp was then resuspended in water (50mL), mixed thoroughly, and centrifuged (10min at 1400 times g), and the supernatant was discarded. Then, the washing process was repeated twice: once with water and then with acetone; the resulting precipitate was dried under vacuum. The obtained dried solid was homogenised by mixing it with a spatula.2.1.2. Isotope Measurements The procedure of isotope ratio mass spectrometry (IRMS) consists in measuring the isotope ratio of an analyte converted into a simple gas, isotopically representative of the original sample, before entering the ion source of an IRMS. The 18O isotopic of the water samples were then analyzed using a stable isotope ratio mass spectrometer IRMS (Delta V Advantage, Thermo Scientific). For ��2H, the equipment used was a Liquid-Water Isotope Analyzer (DLT-100, Los Gatos Research).