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and wrote the manuscript, VC and DY performed the mass spectrometric measurements and the data analyses. RH was responsible for the design of the study and MN participated in the manuscript preparation and revised it critically. All authors read and approved the final manuscript.”
“Introduction Cancer xenograft models of immunodeficient mice are widely applied in various cancer research areas. Recently, xenografted human tumors are commonly used for preclinical drug testing, including biomarker discovery. [1, 2] It has been reported that there is a close correlation between the effects in xenografts buy 5-FU and clinical outcomes, in terms of both drug resistance and sensitivity.  An eventual goal of such preclinical studies using mouse xenograft models is the realization of personalized medicine. Molecular analyses using clinical specimens or xenografted tumors are essential in research for personalized medicine, and high purity samples of sufficient volume are necessary for precise analyses. In general, mouse xenografts are superior to clinical specimens because of the abundance and renewability of the tumor samples. Tumors consist of two components, i.e.