Acknowledgements We are grateful to Dr. P. Desai for the K26GFP virus and Dr. Longnecker for CHO-K1 cells VE821 and HSV-1 (KOS) gL86. We are also indebted to Dr. van der Sluijs for the anti-Rab27a antibody, Dr. M. Izquierdo for the HOM-2 cells, Dr. L. Montoliu for MeWo cell line and Dr. Campagnoni for his kind gift of the HOG cell line. Carlos Sánchez, M. Angeles Muñoz and Verónica Labrador, are also acknowledged for their assistance with the use of the confocal microscope. We are also grateful to Fernando Carrasco, Laura Tabera, Alberto Mudarra and Sandra

Gonzalo, members of the Genomics Core Facility at CBMSO, for their technical assistance. Silvia Andrade is also acknowledged for her technical assistance with flow cytometer and Beatriz García for her technical support. References 1. Noseworthy JH: Progress in determining the this website causes and treatment of multiple sclerosis. Nature 1999, 399:A40-A47.PubMedCrossRef 2. Christensen T: Human

herpesviruses in MS. Int MS j/MS Forum 2007, 14:41–47. 3. Sanders VJ, Waddell AE, Felisan SL, Li X, Conrad AJ, Tourtellotte WW: Herpes simplex virus in postmortem multiple sclerosis brain tissue. Arch Neurol 1996, 53:125–133.PubMedCrossRef 4. Charpin C, Gambarelli D, Lavaut MN, Seigneurin JM, Raphael M, Berard M, Toga M: Herpes simplex virus antigen detection in human acute encephalitis: an immunohistochemical study using avidin-biotin-peroxidase complex method. Acta neuropathol 1985, 68:245–252.PubMedCrossRef 5. Skoldenberg B: Herpes simplex encephalitis. Scand J Infect Dis 1996, 100:8–13. 6. Kastrukoff LF, Lau AS, Kim SU:

Herpes simplex virus type 1 induced multifocal demyelination of the central nervous CH5183284 cost system in mice. Ann N Y Acad Sci 1988, 540:654–656.PubMedCrossRef 7. Kastrukoff LF, Lau AS, Kim SU: Multifocal CNS demyelination following peripheral inoculation with herpes simplex virus type 1. Ann Neurol 1987, 22:52–59.PubMedCrossRef 8. Bello-Morales R, Fedetz M, Alcina A, Tabares E, Lopez-Guerrero JA: High susceptibility of a human oligodendroglial cell line to herpes simplex type 1 infection. J neurovirol 2005, 11:190–198.PubMedCrossRef 9. Mettenleiter TC: Budding events in herpesvirus morphogenesis. Virus res 2004, 106:167–180.PubMedCrossRef 10. Mettenleiter TC, Klupp BG, Granzow H: Herpesvirus Morin Hydrate assembly: an update. Virus res 2009, 143:222–234.PubMedCrossRef 11. Johnson DC, Baines JD: Herpesviruses remodel host membranes for virus egress. Nature rev 2011, 9:382–394.CrossRef 12. Granzow H, Klupp BG, Fuchs W, Veits J, Osterrieder N, Mettenleiter TC: Egress of alphaherpesviruses: comparative ultrastructural study. J Virol 2001, 75:3675–3684.PubMedCrossRef 13. Mettenleiter TC: Intriguing interplay between viral proteins during herpesvirus assembly or: the herpesvirus assembly puzzle. Vet Microbiol 2006, 113:163–169.PubMedCrossRef 14. Murphy MA, Bucks MA, O’Regan KJ, Courtney RJ: The HSV-1 tegument protein pUL46 associates with cellular membranes and viral capsids. Virology 2008, 376:279–289.