We were particularly interested in the difference in chemical compositions between the JAK1/2 inhibito precipitated layer covering the samples and the particles formed on the surface of this layer (Table 1). EDX Ca/P ratio of TCP and TCP-T samples before and after 3 and 8 d of biomimetic study The atomic concentrations of elements and the Ca/P, O/Ca and O(1s)II/O(1s)total atomic ratios obtained from XPS measurements were calculated (Table 2). In comparison, the experimental Ca/P, O/Ca and O(1s)II/O(1s)total ratios measured by Lu et al.18 on synthesized and finely characterized HA, ��-TCP, OCP, MCP, DCP, DCPD and pig cortical bone tablets are grouped in the Table 3.

These values will be useful for distinguishing the six important biologically calcium phosphate phases: HA, ��-TCP, DCPD, DCP, MCP, OCP that could coexist on the TCP�CT samples after 8 d of immersion in CM and NCM fluids under static and dynamic conditions. The values calculated by Lu and al.18 seem really appropriate to calculate our phases since the Ca/P ratio of TCP-T control (Ca/P = 1.23) was closer to Lu et al.��s value (Ca/P = 1.35) than the theoretical value (Ca/P = 1.5). As Lu et al.��s value of Ca/P is slightly different form our experimental Ca/P ratio, all our ratio values were modified by a parameter ��r�� representing the deviation between our control values and Lu and al.18 ones. Moreover, they developed an original method based on the study of the O1s loss spectrum to identify phases that we applied to our samples (see Fig.

5). Table 2. Chemical composition (at.%) and atomic ratios for TCP-T samples in dynamic and static conditions, determinated from XPS analysis Table 3. Direct Ca/P, O/Ca and O(1s)II/O(1s) total ratio given by Lu HB et al.18 Figure 5. O1s loss spectrum of TCP-T ceramic after 8 d in NCM and CM culture media in dynamic conditions. The O1s photoelectric peak is located at 532 eV. The area ratio between the two components of the loss spectrum (554 eV and 568 eV) are modified … The parameter ��r�� for each ratio is calculated as follow: the rCa/P = 1.35/1.23 = 1.097; rO/C = 2.72/2.38 = 1.14 and rO(1s)II/O(1s)total = 0.072/0.056 = 1.28. Table 4 presents the modified values [calculated by multiplying the parameter ��r�� with the initial values of Ca/P, O/C and O(1s)II/O(1s)total ratio].

Ca/P, Dacomitinib O/C and O(1s)II/O(1s)total ratio, calculated for the NCM and CM conditions in static and dynamic experiments, are sensitive to the phases detected at the surface of TCP-T. On the basis of Lu and al.18 values, it is then possible to give a chemical interpretation of the phases present at the extreme surface (9 nm with XPS measurment) of TCP substrates where dissolution and precipitation reaction occurs. Table 4. Identification of CaP phases for TCP-T samples after normalization from values given by Lu et al.