This enzyme is encoded by the thiopurine S-methyltransferase coding gene (TPMT gene). The TPMT locus is subjected to several polymorphisms,

with heterozygous individuals (6%–11% of Caucasian individuals) having intermediate TPMT activity and homozygous mutant individuals (0·2%–0·6% Selinexor research buy of Caucasian individuals) having very low TPMT activity. To date, 20 variant alleles (TPMT*2-*18) have been identified, which are associated with decreased activity compared with the TPMT*1 wild-type allele [28]. More than 95% of defective TPMT activity can be explained by the most frequent mutant alleles TPMT*2 and TPMT*3. The impaired or absent ability to metabolize AZA leads to high blood levels and an increased risk of developing severe and potentially life-threatening myelotoxicity when no dose reductions are performed [29,30].

Dervieux et al.[31], measuring the TPMT activity in red blood cells of paediatric patients after renal transplantation, demonstrated that elevated TPMT activity was associated with an increased risk of acute rejection. Genotyping for TPMT polymorphisms, before initiation of AZA therapy, may be a useful future tool to reduce clinical complications in patients undergoing this treatment. For cyclosporine (CsA) and tacrolimus (TAC), potent agents used widely to treat a variety of autoimmune renal disorders and to prevent acute rejection after renal transplantation, the impact of genetic variability has not yet been defined completely. click here As shown in Table 1,

for phase I metabolism it has already been demonstrated that expression of the multi-drug resistance 1 (MDR-1) gene that encodes for an efflux pomp which removes lipophilic drugs may influence significantly the pharmacokinetics and pharmacodynamics of both CsA and TAC. Regarding phase II metabolism, the relationship between polymorphisms in the P450 cytochrome system, an intracellular transporter system that is capable of carrying a variety of endogenous and exogenous compounds out of the cell, and pharmacological and clinical aminophylline outcomes associated with CNI administration has been evaluated in several reports (Table 1). In particular, it has been reported that patients carrying CYP3A5*3, an allelic variant of the CYP3A5 gene that results in lack of enzyme expression, reaching high dose-adjusted levels, need lower dosages of CNIs compared to those with the wild-type genotype (CYP3A5*1/*1) [32,35,37,41–43,46,47,49,54,55]. However, the contribution of additional polymorphisms needs to be evaluated more clearly and addressed in future pharmacogenetic studies. The metabolism of MPA, a selective inhibitor of the de novo purine synthesis via inosine monophosphate deydrogenase (IMPDH) enzyme inhibition, is also influenced largely by several genetic polymorphisms (Table 1).