The synthetic conditions were optimized by a comprehensive investigation on raw materials consumption, size distribution, and surface morphology. Preparation of microcapsules with high wrap ratio was also demonstrated. The as-synthesized microcapsules were studied using various characterizations techniques, including optical microscope, fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning CB-5083 manufacturer calorimetry, and contact angle meter. Spherical microcapsules (size: similar to 60 mu m) with smooth surface were obtained when the stirring rate was 400 rpm and the amount of
core materials is 76 wt %. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 115: 2162-2169, 2010″
“Introduction: Early randomized clinical trials of autologous bone marrow cardiac stem cell therapy have reported contradictory results highlighting the need for a better evaluation of protocol designs. This study was designed to quantify and compare whole body and heart cell distribution after intracoronary or peripheral intravenous injection of autologous bone marrow mononuclear cells in a porcine acute myocardial infarction model with late reperfusion.
Methods: Myocardial infarction was induced using balloon inflation in the left coronary artery in domestic pigs. At seven days post-myocardial infarction, 1×10(8) autologous bone marrow
mononuclear cells were labeled with fluorescent marker and/or(99m)Tc radiotracer, and delivered using intracoronary or peripheral intravenous injection (leg vein).
Results:
Scintigraphic analyses selleck chemicals SNX-5422 research buy and.-emission radioactivity counting of harvested organs showed a significant cell fraction retained within the heart after intracoronary injection (6 +/- 1.7% of injected radioactivity at 24 hours), whereas following peripheral intravenous cell injection, no cardiac homing was observed at 24 hours and cells were mainly detected within the lungs. Importantly, no difference was observed in the percentage of retained cells within the myocardium in the presence or absence of myocardial infarction. Histological evaluation did not show arterial occlusion in both animal groups and confirmed the presence of bone marrow mononuclear cells within the injected myocardium area.
Conclusions: Intravenous bone marrow mononuclear cell injection was ineffective to target myocardium. Myocardial cell distribution following intracoronary injection did not depend on myocardial infarction presence, a factor that could be useful for cardiac cell therapy in patients with chronic heart failure of non-ischemic origin or with ischemic myocardium without myocardial infarction.”
“The kinetics of photodegradation of levofloxacin in solution on UV irradiation in the pH range 2.0-12.0 has been studied using a HPLC method.