Our laboratoryhas prevously showthat MnSOD s susceptble to tyrosne ntratoand oxdatowhch leads to nactvatoof the enzyme,hence addtonal oxdant producton.These reviews obviously demonstrated that reduction of MnSOD protedd not account for reduction of enzymatc actvty durng renal transplant njury,rather submit translatonal modfcatons within the enzyme were nvolved.addton, these studes also showed that MnSOD nactvatopreceded renal damage even more suggestng that reduction of MnSOD actvty was a major event renal injury followng schema.even so, the mechanstc pathways nvolved wth the protectogoverned by MnSOD remalargely unknown.Thshas encouraged us to nvestgate the molecular occasions downstream for the lowered expressoof MnSOD enzyme wththe kdney usng avvo model.The growth “selleck “ of transgenc and gene KO mce whch the MnSOD gene s ether over expressed or knocked out, respectvely, provdes a robust instrument to research the consequence of decreased MnSOD dsease and or to determne the enzymes contrbutory function typical physology.
however, as mentoned earler, the complete MnSOD KO mce de, and theheterozygous partal MnSOD KO mce exhbt decreased MnSOD actvty all tssues organs whch makes nterpretatoof these data ospecfc orgafunctoless clear.Consequently, the objective of ths study was to develoa novel mouse model that mmcs a condtoof renal nactvatoof MnSOD vvo.Ths paper descrbes the generatoof a kdney specfc MnSOD KO mouse lne usng Cre medated deletoof MnSOD AZD5438 allele.These renal specfc KO mce wl serve as anvaluable tool to investigate the molecular mechansms that take place downstream of MnSOD nactvatovarous renal dsorders and could possbly be helpful other pathologes that depend onormal renal functon.Materals and Strategies Mce Two transgenc mouse lnes have been applied to develokdney specfc MnSOD KO mce.The frst transgenc mouse lne expressed Cre recombnase specfcally the kdney and was a generous gft from Drs.Peter garash and Paul Overbeek.Ths mouse lne s oa C57BL six background.The second transgenc mouse lne was the MnSOD floxed mouse oa C57BL6CrSlc background, whch was a generous gft from Drs.
Takuj Shrasawa and Tng Tnghuang.Mce have been mantaned accordng for the crtera outlned the Gude for your Care http://t.co/MfAIst4oCe

— Lasyaf Hossain (@lasyafhossain) November 8, 2013

and Use of Laboratory Anmals publshed by the Natonal nsttutes ofhealth.All of your anmal protocols had been approved by the nsttutonal Anmal Care and Use Commttee at the Unversty of Arkansas for Medcal Scences to perform as descrbed the paper.Generatoof mce wth kdney specfc deletoof MnSODheterozygous female MnSOD floxed mce had been crossed wthheterozygous male Kdney Cre mce that express Ksp1.3 Cre transgene specfcally the kdney as lustrated Fgure 1B.From the fal 1 progeny, mce wthheterozygous deletoof MnSOD gene thatharbor Ks1.3 Cre transgene have been selected.