It is not clear whether the clearance of insulin is altered in liver steatosis. Methods: This study was designed to observe the change of expression of
IDE in C57BL/6 mice with high fat diet-induced liver steatosis. Liver steatosis was induced in C57BL/6 mice via feeding with high fat diet for 16 weeks and the mice with chow diet as the control group. The hepatic protein and mRNA level of IDE were determined by western blot and RT-PCR. To make a compare, the intensity of the protein signal was analyzed quantitatively using Image J software. Results: Macroscopic and microscopic findings demonstrated that lipids were accumulated in the liver and liver steatosis was confirmed. Western blot showed that IDE was obviously higher in the high fat diet group that that in the control group (1 ± 0.17 vs 2.80 ± 0.24, p < 0.05). But the relative IDE mRNA level Alpelisib clinical trial of the high fat diet induced
MG-132 clinical trial steatosis group was significantly lower than those in the control group (1 ± 0.09 vs 0.35 ± 0.05, p < 0.05). The decreased expression of IDE mRNA may be a compensation for the increased expression of IDE protein level. Conclusion: IDE is increased in mice with high fat diet induced liver steatosis. Key Word(s): 1. insulin degrading; 2. liver steatosis; Presenting Author: LILI YUAN Additional Authors: RUI ZHANG, NA ZHU, PING CAO Corresponding Author: LILI YUAN Affiliations: MCE公司 Shanxi Dayu hospital Objective: There is a need for
us to get some effective and noninvasive methods to detect liver steatosis, which is a factor of liver fibrosis. Ultrasonic controlled attenuation parameter (CAP) is devised to target liver steatosis, which is based on vibration control transient elastography (VCTE). In this work, liver steatosis is evaluated using the novel CAP. Methods: All 60 patients were received examinations of liver Ultrosound, serum liver enzymes, and Fibroscan for measurement of transient elastography (TE) and CAP. Literature shows E value of Fibroscan significantly correlated with liver fibrosis. Grades of steatosis were divided by four groups (S0, S1, S2 and S3) by using Fibroscan CAP, 245,299 and 321 were the cutoff values of S1, S2 and S3. Mild and moderate to severe fatty liver were divided by using Ultrasound. Results: With the grades of steatosis progress diagnosed by Fibroscan CAP, the CAP value was significantly elevated in late groups compared with their previous groups respectively (table 1, p < 0.05), but there is no difference between the two groups diagnosed by Ultrasonic. No correlation was found for liver enzymes with grade of steatosis diagnosed by both methods. The E value was significantly increased in S3 group compared with in S0, S1 groups (table1, p < 0.05), indicted that with liver steatosis worsen, liver fibrosis was progressed.