Infect Immun 1994, 62:2440–2449.PubMed Authors’ contributions MAU carried out the microarray experiments and the bioinformatics analyses, and participated in the analysis of the data and drafting of the manuscript. GHLC designed the microarray experiments, and participated in analysis of the data and CHIR98014 solubility dmso drafting of the manuscript. JFFG participated in and supervised drafting of the manuscript. FMS participated in and supervised the design of the microarray experiments and the analysis of the microarray data. AG participated

in the design of the study and drafting of the manuscript. LD participated in the design of the study. MB conceived the study, and participated in its design and coordination. All the authors read and approved the final manuscript.”
“Background Fonsecaea AZD2171 nmr pedrosoi is a soil-borne dimorphic fungus and the major EPZ015666 solubility dmso etiological agent of chromoblastomycosis, a chronic disease that can affect immunocompetent hosts. F. pedrosoi is usually limited to skin tissue, most commonly on the lower limbs. Infection

usually occurs after exposure to the fungus via contaminated soil, splinters or sharp farm equipment, and results in long-term inflammation, suppurative granulomatous dermatitis and fibrosis [1, 2]. The affected patients are typically low-income workers that engage in agricultural or manual labour in tropical and subtropical countries. Rarely, F. pedrosoi can also cause phaeohyphomycosis, in immunosuppressed patients [3]. The management of diseases caused by F. pedrosoi O-methylated flavonoid continues to be challenging. Treatment depends on an early diagnosis and

the use of systemic antifungal agents and local therapies, including the surgical removal of lesions. The suggested drug interventions are expensive, involving high doses of itraconazole and/or terbinafine (200 to 400 mg and 250 to 500 mg, respectively) daily for over one year. Even with treatment, relapses are common [4, 5]. F. pedrosoi constitutively produces melanin [6], a pigment that is an important virulence factor in several human pathogenic fungi due to its anti-oxidative, thermostable, anti-radioactive, paramagnetic and metal binding properties. Melanins are present in both prokaryotic and eukaryotic organisms. These ubiquitous dark compounds are formed by the oxidative polymerisation of phenolic or indolic compounds. Melanins have been extensively studied and characterised as negatively charged amorphous compounds with quinone groups, hydrophobic and insoluble in organic solvents [7, 8]. Efforts to elucidate the structure of melanins are not yet conclusive due to limitations of the biochemical and biophysical analytical methods available. Electron spin resonance (ESR) can characterise pigments, including melanin, and reveals that a typical melanin spectrum falls between 3300 and 3500 gauss [7–9]. Franzen et al. [10, 11] reported that F.