Increased concentrations of these compounds produce symptoms and allow for clinical evaluation of disease. AZD9291 Historically, screening for such tumors by determination of catecholamines and metabolites in urine
yielded false negative results in individuals with a genetic predisposition for the disease and those with paroxysmal hypertension. Analysis of metanephrines in plasma, however, is of decisive diagnostic importance. This test exhibits high sensitivity and specificity for the analytes produced by tumors.\n\nMethods: Plasma proteins are removed by solid phase extraction. Chromatographic isolation of the analytes and stable isotope internal standards is achieved by elution on a HILIC column
connected to a UPLC MS/MS system. Metanephrines are measured using multiple reaction monitoring with an electrospray source operating in positive ion mode.\n\nResults: The method was validated for linearity, limit of quantification, accuracy, and precision. The method was accurate and correlated well to a comparison HPLC method. Potential interferences were evaluated.\n\nConclusions: Results from this LC-MS/MS assay enable clinical diagnosis of pheochromocytoma and aid in monitoring treatment outcomes. (C) 2012 Elsevier B.V. All rights reserved.”
“P.L. PRIETO-HONTORIA, P. PEREZ-MATUTE, M. FERNANDEZ-GALILEA, A. BARBER, J.A. MARTINEZ and M.J. BAY 1895344 price MORENO-ALIAGA. Lipoic acid prevents body weight gain induced by a high fat diet in rats: effects on intestinal sugar transport.
J Physiol Biochem, 65 (1), 43-50, 2009.\n\nSeveral studies have suggested that oxidative stress might cause and aggravate the inflammatory state associated with obesity and could be the link between excessive weight gain and its related disorders such as insulin resistance and cardiovascular diseases. Thus, antioxidant treatment has been proposed as a therapy to prevent and manage obesity and associated complications. Therefore, the aim of the present study was to investigate the effects of supplementation of a standard or high G418 Microbiology inhibitor fat diet with the antioxidant lipoic acid (LA) during 56 days, on body weight gain, adiposity, feed efficiency and intestinal sugar absorption, in male Wistar rats. LA supplementation induced a lower body weight gain and adipose tissue size in both control or high fat fed rats accompanied by a reduction in food intake. The group fed on a high fat diet and treated with LA (OLIP group) showed a lower body weight gain than its corresponding Pair-Fed (PF) group (P < 0.05), which received the same amount of food than LA-treated animals but with no LA. In fact, LA induced a reduction oil feed efficiency and also significantly decreased intestinal alpha-methylglucoside (alpha-MG) absorption both in lean and obese rats.