guilliermondii JAK drugs from M. caribbica and other species of M. guilliermondii complex during in silico restriction digestion of the ITS1-5.8S-ITS2 amplicon sequences. (PDF 241 KB) Additional file
2: Figure S1: Neighbour-joining phylogenetic tree based on LSU rRNA gene D1/D2 sequences showing taxa-nonspecific segregation of M. guilliermondii strains. The tree was constructed based on the evolutionary distance calculated using Kimura-2 parameter from the representative nucleotide sequences of M. guilliermondii and M. caribbica (position 13 to 308 of LSU rRNA gene of S. cerevisiae CBS 1171, GenBank Accession No. AY048154.1). The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to selleck chemicals llc the branches. The bar represents 1% sequence divergence. GenBank accession numbers are mentioned within the parentheses. S. cerevisiae was the outgroup in the analysis. T = Type strain. Figure S2. In silico identified restriction enzymes which distinctly differentiated M. guilliermondii from M. caribbica. Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of various strains of the two species obtained from
NCBI GenBank and CBS yeast database showing position of identified ArsI (A), BfaI (B), BsrI (C), Hpy188I (D), HpyCH4III (E), and MmeI (F) restriction recognition sites (highlighted) which distinctly differentiated the two species. The nucleotide position was based on the sequence of the in silico PCR amplicon of Alanine-glyoxylate transaminase ITS1-5.8S-ITS2 of S. cerevisiae strain S288c (NC_001144) including gaps generated during multiple sequence alignment. C. fermentati is the anamorph of M. caribbica.
T = Type strain. Figure S3. In silico restriction digestion profile of M. guilliermondii and M. caribbica ITS1-5.8S-ITS2 amplicon. The theoretical restriction digestion profile was generated using NEBcutter, version 2.0 (http://tools.neb.com/NEBcutter2/). Lane G: M. guilliermondii ATCC 6260; Lane C: M. caribbica CBS 9966; Lane M: 100 bp DNA ladder. Figure S4. Strain level diversity of M. guilliermondii revealed by PFGE karyotyping. Lane 1 − 13: Isolates A3S2Y1, Kw1S2Y1, Kw3S3Y1, A3S6Y1, A2S6Y1, A1S9Y1, A1S9Y5, A2S9Y1, A3S9Y1, A3S9Y9, A2S10Y1, A2S10Y4 and A3S11Y1. White arrow indicates the polymorphic chromosomal band. (PDF 298 KB) References 1. Dujon B: Yeast evolutionary genomics. Nat Rev Genet 2010, 11:512–524.PubMedCrossRef 2. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP: Candida Berkhout (1923). In The Yeasts: A Taxonomic Study, Volume 2. 5th edition. Edited by: Kurtzman CP, Fell JW, Boekhout T. San Diego: Elsevier; 2011:987–1278.CrossRef 3. Lan L, Xu J: Multiple gene genealogical analyses suggest divergence and recent clonal dispersal in the opportunistic human pathogen Candida guilliermondii . Microbiology 2006, 152:1539–1549.PubMedCrossRef 4.