, 2012) but underwent

additional experimental manipulations for the http://www.selleckchem.com/products/fg-4592.html present work, and two additional rats were used exclusively for this study. The mean percentage of correct trials increased greatly over the course of learning, following a standard learning curve (Figure 1C). There was an initial phase of rapid improvement followed by a phase of slower learning, representing early (days 2–4) and late (days 8–11) learning. The percentage of correct trials increased significantly from early to late in learning (p < 0.001), demonstrating that rats were able to properly learn the task. Analyses of M1 firing rates further showed that rats were producing the desired ensemble rate modulations during task performance (Figure 1B). We first investigated the relationship between spiking activity and the LFP oscillations recorded during task engagement. We performed spike-triggered averaging of the LFP in late learning time locked to spikes occurring either in the same region or in the other region. If spiking activity was independent of LFP phase, then fluctuations would cancel and produce a flat average LFP. Instead, we observed clear mean LFP oscillations in both regions around action potentials from both regions; this oscillatory activity

had a strong component between 6–14 Hz (Figure 2A). This is consistent with past work showing that oscillations in this range are prominent in corticostriatal circuits when performing well-learned tasks (Berke et al., 2004), as well as work suggesting that M1 is predisposed to operate in this frequency range (Castro-Alamancos, 2013). We BLZ945 therefore filtered the raw LFP from 6–14 Hz and calculated the predominant phase at which spikes occurred. Again, we observed clear phase locking of spikes to the ongoing 6–14 Hz LFP in both regions (Figure 2B). Although the relationship between LFP and spiking is certainly complex and cells spike at several preferred LFP phases, there was nevertheless

a dominant phase preference across both regions. about Interestingly, both DS and M1 spikes occurred preferentially at the peak of the striatal 6–14 Hz LFP oscillation, suggesting that DS firing is maximal at the peak of the DS LFP. To further quantify these interactions and the ways they evolve during learning, we calculated coherence between spiking activity in M1 and LFP oscillations in DS. We analyzed 1,936 spike-field pairs (121 M1 units and 16 DS LFP channels). To avoid effects of evoked responses on coherence estimates, we subtracted the mean DS event-related potential (ERP) and M1 time-varying firing rate for each cell or LFP channel, respectively, from individual trials before calculating coherence (Figure S2). We saw a profound increase in spike-field coherence across a range of low frequencies in late learning, when rats were skillfully performing the task, relative to early learning (Figure 2C).