02* 1.19* 1.21* Francci3_0114 phage integrase -1.10* 1.54 1.70 Francci3_0407 phage integrase 1.48 1.23 -1.20 Francci3_0878 phage integrase 1.05* 1.55 1.48 Francci3_1095 phage integrase 1.46 1.62 1.11 Francci3_1144 phage

integrase 2.72 1.63 -1.67 Francci3_1203 phage integrase 1.39 1.66 1.20 Francci3_1870 phage integrase-like SAM-like 3.05 1.53 -2.00 Francci3_2053 phage integrase-like SAM-like -1.32 1.83 2.43 Francci3_2147 phage integrase 1.92 1.52 -1.26 Francci3_2228 phage shock protein A, PspA 2.47 1.43 -1.73 Francci3_2304 phage integrase 1.60 -1.24* -1.99 Francci3_2344 phage integrase 1.59 1.20* -1.32 Francci3_2443 putative phage-related terminase large subunit 1.34 1.84 1.37 Francci3_2954 bacteriophage (phiC31) resistance gene PglY

1.57 Elafibranor molecular weight 1.38 -1.14* Francci3_2955 bacteriophage (phiC31) resistance gene PglZ 1.47 1.22* -1.21* Francci3_3052 phage integrase 1.07* 1.43 1.34 Francci3_3350 phage integrase 1.42 1.74 1.22 Francci3_3388 phage integrase 1.55 1.84 1.19 Francci3_3390 phage integrase 1.89 -1.09* 1.73 Francci3_3532 phage integrase 2.02 1.48 -1.36 Francci3_3535 phage shock protein A, PspA -1.98 -1.86 1.06* Francci3_3583 phage integrase -1.34 1.39 1.86 Francci3_3734 phage integrase-like SAM-like 1.34 1.62 1.21 Francci3_4274 phage integrase 4.52 1.60 -2.83 Francci3_4338 phage integrase -1.36 1.69 2.30 1Fold changes calculated as quotients of RPKM values *Insignificant p value as determined by Kal’s ztest. Negative values indicate a fold reduction of expression in the reference Atorvastatin (later) MK-4827 solubility dmso condition. CcI3 has four putative CRISPR arrays, two of which are located near clusters of CAS ORFs (data obtained from CRISPRFinder [36]). Three of the CRISPR arrays had high numbers of repeat copies (38, 15 and 20 spacers per array ordered with respect to the OriC) making alignment

of ambiguous sequence reads difficult. Even the shorter 36 bp read lengths of the 5dNH4 sample could not be reliably mapped across the arrays using the CLC Genome Workshop MK1775 alignment programs. As a result, few reads mapped to the array region of the CRISPR islands and numerous deletions were predicted (Additional Files 2 through 7). The CAS ORF transcripts, by contrast, were detected in all three samples. Again, transcription was modestly higher in the 5dNH4 sample than in the 3dNH4 sample (Table 5). In this instance, the 3dN2 sample had nearly two fold higher expression of all CAS ORFs when compared with the 3dNH4 sample. Comparison of the 5dNH4 and 3dN2 samples revealed insignificant fold changes as determined by a Kal’s ztest. Table 5 Fold changes of CRISPR associated ORFs1 Feature ID Annotation 5dNH4 vs 3dNH4 3dN2 vs 3dNH4 3dN2 vs 5dNH4 Francci3_0017 CRISPR-associated helicase Cas3, core 1.31 1.39 1.06* Francci3_0020 CRISPR-associated protein, CT1975 2.99 1.63 -1.84 Francci3_0021 CRISPR-associated protein, CT1976 2.79 1.42 -1.