0 ST Arrays. Manufacturers instructions were followed for the hybridization, Sunitinib c-Kit washing, and scanning steps. Pre labelled spike in controls, unlabelled spike controls, and back ground probes Inhibitors,Modulators,Libraries were included in the analysis. All the microarray data are available at Gene Expression Omni bus. Processing of microarray data Statistical analysis of the microarray Inhibitors,Modulators,Libraries data was performed using Partek Genomic Suite Software. RMA background correction of raw microarray data and normalization of expression values were performed using Partek Genomic Suite Software. Fold changes of expression values were calculated as the ratio of the mean RMA corrected expression value in the hypoxic group to the normoxic group. Fold change values 1 were converted to the nega tive of the inverse ratio.

Hypoxic and normoxic samples were compared using the paired Students t test. The false discovery rate was set to 5% to correct for multiple testing. In the case of subgroup analyses, the threshold was set to P 0. 005. A gene was considered modulated when at least one of the corresponding probe sets showed significantly Inhibitors,Modulators,Libraries different expression levels after correction for multiple testing with a minimal two fold change. Meta analysis of lung cancer transcriptome studies Expression values for the genes of interest were obtained from four eligible lung cancer datasets published at Gene Expression Omnibus. Details on data processing and patient characteris tics are reported at GEO and in the cited literature. Details on data retrieval are indicated in Additional file 1.

Statistical analysis Meta analysis of the effect of MME on patient survival after surgery was performed with a proportional haz ards model with Gaussian random Inhibitors,Modulators,Libraries effects using the package coxme 2. 1 3 of R 2. 13. 2 statistical software. For details see Additional file 1. All other data were compiled and analyzed with the SPSS software package, version 18. 0. Group differ ences were calculated with the paired Students t test, one sample Students t test, Mann Whitney U test, or Wilcoxon signed rank test as applicable. P values smaller than 0. 05 were considered significant. Results Apoptosis and hypoxia markers in NSCLC fragments NSCLC tissue was fragmented immediately after surgery. Fragments were maintained in culture medium for three days, both in ambient oxygen or hypoxia. The lar gest diameter measured from paraffin sections was 1.

Inhibitors,Modulators,Libraries 19 mm, the smallest diameter was 0. 8 mm. There was no significant difference between the size of frag ments cultured in normoxia or hypoxia. The histomorphology of cultured NSCLC fragments resembled the selleckbio growth patterns usually found in freshly resected NSCLC tissue. Cancer cell nests were found in close prox imity to stroma rich regions with only scattered tumor cells. Tumor cells were found in the vast majority of cul tured fragments, large necroses were rare.