[14] genetic map that did amplify as expected in the synthetic. Sequence discrepancy between the different species may be a likely reason for failure of

expression of some SSR bands in the hexaploid hybrid. The SSR primers used were based on sequences of G. arboreum (A1 genome), G. raimondii (D5 genome) and G. hirsutum (A1D1 genome). Compared to these species, many base substitutions may have occurred in the flanking sequences adjacent to the SSR loci in G. anomalum (B1 genome); some of these substitutions may have been in Selleck SB203580 the marker binding sites thus causing a preferential primer binding to genomic DNA from G. hirsutum. As a result, the specific SSR bands of G. anomalum would be undetectable in the hybrid plants. This explanation was confirmed by the observation that amplification of specific SSR bands of G. anomalum also failed when a DNA pool from G. hirsutum and G. anomalum was used instead of synthetic DNA as the template ( Fig. 3). The present results demonstrate the hybridity and doubled status of (G. anomalum × G. hirsutum) using morphological, cytological and molecular marker methods. These materials can be used as bridges for the transfer of useful agronomic traits from the wild species to cultivated varieties. The Crizotinib mw 349 informative SSR markers generated for the

interspecific hybrids can be used to track the flow of genetic material from G. anomalum during backcrossing to G. hirsutum. This work was supported by the National Natural Science Foundation of China (31171595)

and the Independent Innovation Funds for Agricultural Technology of Jiangsu Province, China [CX (12)5039]. “
“Cotton (Gossypium spp.) is one of the most important fiber crops in the world and serves as a source of oil and biofuel [1]. Verticillium wilt has worldwide distribution Verteporfin ic50 and causes serious economic losses [2]. The disease is caused by the soilborne fungus Verticillium dahliae Kleb. The fungus infects the roots of the cotton plant in the soil by entering through cortical cells. Once inside, the spores and mycelia of the pathogen block the vessels of the plant. V. dahliae toxins and acidic glycoproteins are also important pathogenicity factors that can rapidly induce wilting [3]. Strains of the pathogen in China can be divided into two types according to their virulence: defoliating and non-defoliating [4]. An early symptom seen in the host plant after infection by a defoliating pathogen is downward curling and epinasty of the terminal leaf, followed by epinasty of most of the other leaves. These epinastic leaves then exhibit general chlorosis, which eventually leads to defoliation. If cotton plants are infected with a non-defoliating pathogen, the lower leaves exhibit interveinal chlorosis that leads to necrosis, but there is little or no epinasty and any dead leaves usually remain attached to the plant [4]. The main factors affecting the virulence of Verticillium wilt in cotton are the V.